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Participating Countries:

Algeria

Argentina

Australia

Austria

Belgium

Bosnia and Herzegovina

Bulgaria

Croatia

Czech Republic

Denmark

Finland

France

FYR of Macedonia

Germany

Greece

Iceland

Ireland

Israel

Italy

Lithuania

Morocco

Netherlands

New Zealand

Poland

Portugal

Romania

Russian Federation

Serbia

Slovenia

Spain

Sweden

Switzerland

Turkey

Ukraine

United Kingdom

United States

Member area provided by LTFE
COST is supported by the EU Framework Programme Horizon 2020
This website is supported by COST

CNR-IFT National Research Council-Institute of Translational Pharmacology

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Via Fosso del Cavaliere 100
00133 Rome
Italy

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Brief presentation of the institution

The group leader of the Laboratory of Molecular Pathology and Experimental Oncology is involved in studies about electrogene transfer and its medical applications since 2000. The laboratory belongs to CNR-IFT whose research activities focus on mechanisms of insurgence of human disorders particularly of neoplastic, immuno-degenerative, infective and neurogenetic diseases, and on the development of innovative preventive/therapeutic strategies, also considering the translation to the clinic. In this view, the aim of the Laboratory of Molecular Pathology and Experimental Oncology is to develop translational molecular medicine studies for novel target therapies to pass from bench to bedside. The main project led in the lab is the CNR ME P06.031.002 "Innovative methods in Molecular Medicine: development of therapeutic molecules and in vitro and in vivo delivery by electric fields". The group is also involved in education of undergraduate and PhD students.

Main Electroporation related topics

Main research activities of the Laboratory are focused on pre-clinical protocols based on electrotransfer, conducted in models of human diseases: intramuscular naked DNA vaccination, systemic delivery of proteins, electrochemotherapy. Because the stimulation of the immune system and the improvement of the vaccine delivery are crucial points to consider in DNA vaccination protocols, our group is also addressing its studies on the cellular and biochemical events occurring after electrotransfer, exploring the adjuvant properties of this technique and the effects of hyaluronidase pre-treatment for potentiating the DNA intake in muscle cells. The studies are carried on in collaboration with national and international research groups.

Main Electroporation related research techniques and equipment

In vitro and in vivo electrotransfer protocols by BTX ECM830 (Harvard Apparatus) equipped with TweezertrodesTM, Needle ArrayTM kit, Petri Pulser Electroporation ApplicatorTM

Cellular and Molecular Biology facilities: cell culture hoods and incubators, laminar flow chambers, cooled centrifuges, minifuges, spectrophotometer Beckman, electrophoresis apparatus, microscopes (light, fluorescent), real time PCR, Victor3 PerkinElmer, Versadoc.

Research topics, techniques and equipment not related to Electroporation

The research activity is also focused on the molecular mechanisms in the development and progression of inflammatory and tumoural diseases, in the identification of biomarkers, in antigen selection and optimization, in adjuvant molecules.

Brief information on available facilites

laboratory for molecular biology

laboratory for cellular biology

Willingness to accept/host visiting researchers

Publications

Project Office

Working groups

Steering Committee

Founding members

DC Rapporteurs

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