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Participating Countries:

Algeria

Argentina

Australia

Austria

Belgium

Bosnia and Herzegovina

Bulgaria

Croatia

Czech Republic

Denmark

Finland

France

FYR of Macedonia

Germany

Greece

Iceland

Ireland

Israel

Italy

Lithuania

Morocco

Netherlands

New Zealand

Poland

Portugal

Romania

Russian Federation

Serbia

Slovenia

Spain

Sweden

Switzerland

Turkey

Ukraine

United Kingdom

United States

Member area provided by LTFE
COST is supported by the EU Framework Programme Horizon 2020
This website is supported by COST

University of Twente

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Postbus 217
7500 AE Enschede
Netherlands

Contact email:

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Brief presentation of the institution

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Main Electroporation related topics

Research in our group focuses on the advantages brought by miniaturized and microfluidic approaches to enhance the success rate of electroporation and electrofusion. A former project examined single cell electroporation and gene transfer in individual cells trapped in a microfluidic device using integrated and independently addressed electrodes. As a direct continuation, more fundamental work was performed to get a better insight into poration processes using planar models for cell membranes. Currently, a similar approach is adopted to electrolyse individual cells for their molecular characterization, with a particular focus on cancer cells and analysis of their genetic content.Next to this, microfluidic protocols are developed for cell electrofusion using trapping sites or droplet platforms.

 

Main Electroporation related research techniques and equipment

- Fluorescence microscopes equipped with cameras (incl. high speed and high sensitivity cameras)

- Flow controllers for microfluidic experimentation (Harvard syringe pumps, Nemesys syringe pumps, Fluigent systems, Elvesys pumps)

- Wave generators

- Oscilloscopes

- Power source, HV supply

- Impedancemeter

- Equipment for cell culture (incubators, centrifuges, autoclave, laminar flow chambers, cell counting systems, etc)

- Equipment for device fabrication using soft lithography and hot embossing

Research topics, techniques and equipment not related to Electroporation

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Brief information on available facilites

- Cleanroom (Nanolab) of the MESA+ Institute for Nanotechnology

- Cell culture facility

- Bionanolab of the MESA+ Institute for Nanotechnology (incl. pipetting robots, confocal microscopy, Raman confocal microscopy, nano-spotter, liquid AFM, etc.)

Willingness to accept/host visiting researchers

Members:

  • Séverine Le Gac
    Email:

    Position: Researcher
  • Sertan Sukas
    Email:

    Position: Researcher

Project Office

Working groups

Steering Committee

Founding members

DC Rapporteurs

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