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Algeria

Argentina

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FYR of Macedonia

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Member area provided by LTFE
COST is supported by the EU Framework Programme Horizon 2020
This website is supported by COST

Wroclaw University of Technology, Institute of Biomedical Engineering and Instrumentation

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Wybrzeze Wyspianskiego 27
50-370 Wroclaw
Poland

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Brief presentation of the institution

The group of Bioinformatics and Biophysics of Nanopores, founded by Prof. Malgorzata Kotulska, is a part of the Institute of Biomedical Engineering and Instrumentation of Wroclaw University of Technology. The members of our group are involved in research and education in the field of biophysics, biochemistry and bioinformatics. We are focused on structure and function of biological nanopores, such as electropores and protein ion channels.
Experimental group conducts research on electroporation as a potential drug delivery system. Mechanism of electroporation is studied on a planar lipid bilayer model. The possibility of application of electroporation as a support for anti-cancer therapies is investigated in vitro.
Our group, in collaboration with Wroclaw Medical University, is a leading electroporation-oriented team in Poland.
Part of our group is focused on bioinformatics, especially on protein structure prediction and models validation. Machine learning methods, ontological databases and advanced techniques of protein modeling are used in theses studies.

Main Electroporation related topics

  • experimental research on electropores induced in planar lipid membranes – conductance characteristics and structure-function relation (BLM model)
  • electroporation for drug delivery (in vitro model)
    - photodynamic therapy combined with electroporation
    - electrochemotherapy

Main Electroporation related research techniques and equipment

  • system designed for electroporation under constant-current conditions
  • system designed for BLM formation
  • square wave pulse generator designed for in vitro electroporation (equipped with a stand for cuvettes or different electrodes)
  • differential fluorescence detector
  • oscilloscopes

Research topics, techniques and equipment not related to Electroporation

  • computer modeling of ion channels and amyloidogenic proteins
  • protein structure prediction and model validation (bioinformatics and machine learning methods)

Brief information on available facilites

Present in our group:

  • biophysical laboratory with facilities for electroporation studies on BLM model with access to the institute laboratory equipment
  • facilities for computation, simulation, and programming

 Present in our institute:

  • microscopic facility (atomic force microscope, confocal microscope, Kelvin Probe microscope, fluorescence microscope, optical microscope with digital camera)
  • spectroscopic facility ( spectrophotometers, spectrofluorimeters)
  • biophysical facility (balances, ph-meter, conductometer, centrifuge, ultrasound bath, magnetic stirrer, water-puryfying system, fume hood, incubator)

Willingness to accept/host visiting researchers

Publications

Projects:

  • COST TD1104: European network for development of electroporation-based technologies and treatments (EP4Bio2Med) (2011-2015)
    Project type: International
    Funding: European Commission, Brussels, Belgium
  • Machine learning methods for protein contact sites prediction ( Nr 6435/B/T02/2011/40, 2011-2014)
    Project type: National
    Funding: National Science Center, Poland
  • Quality assessment of transmembrane protein structural models using Brownian Dynamics and energy models – correlation between functional and other measures (Diamond Grant 2012-2015)
    Project type: National
    Funding: Ministry of Science and Higher Education, Poland

Members:

  • Witold Dyrka
    Email:

    Position: Researcher
  • Bogumil Konopka
    Email:

    Position: PhD student
  • Malgorzata Kotulska
    Email:

    Position: Professor
  • Monika Kurczynska
    Email:

    Position: PhD student
  • Joanna Wezgowiec
    Email:

    Position: PhD student

Project Office

Working groups

Steering Committee

Founding members

DC Rapporteurs

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