Special Issue on Electroporation-Based Technologies and Treatments
The Journal of Membrane Biology
Volume 248, Issue 5, October 2015
Issue Editors: Damijan Miklavcic, Lluis M. Mir, P. Thomas Vernier
Special Issue on Electroporation-Based Technologies and Treatments
This special issue of the Journal of Membrane Biology contains reports on recent developments in the field of electroporation by participants in the International Workshop and Postgraduate Course on Electroporation-Based Technologies and Treatments held in November 2014 in Ljubljana. This was the eighth edition of what is now an annual event, first organized in 2003. The 66 participants—students, young scientists, invited lecturers, faculty members, and special guests—came from 15 countries (Algeria, Belgium, China, Croatia, France, Germany, Ireland, Israel, Italy, Lithuania, Macedonia, Netherlands, Slovenia, Spain, and United States). In addition to lectures on the physics, electrical engineering, and biology of electroporation, this year’s sessions included topical talks on electrochemical processes associated with pulsed electric field exposures, electroporation in food and biomass processing, systemic electrochemotherapy, and electrochemotherapy of colorectal liver metastases. One of the features of this week-long workshop course is the practical training offered each afternoon. Organized and led by associates of the Laboratory of Biocybernetics of the Faculty of Electrical Engineering at the University of Ljubljana, and by visiting researchers, these laboratory sessions provide a unique opportunity for hands-on experience guided by experts, with activities including gene electrotransfer, ratiometric fluorescence microscopy, microbial inactivation, electrical characterization of artificial membranes, treatment planning for electrochemotherapy, and molecular dynamics simulations. The school is now an established and unique platform for the acquisition of theoretical and practical knowledge of electroporation mechanisms and applications.
The peer-reviewed selection of articles in this issue provides a cross section of the ongoing electroporation related research being carried out by participants at the Ljubljana meeting. We are grateful to the contributors for their efforts in presenting their recent results, which will challenge readers, whether new or old in the field, to evaluate assumptions and consider new ideas, and we are especially grateful to our scientific colleagues who reviewed the manuscripts.
The workshop and postgraduate course were conducted within the scope and with the support of the European Associated Laboratory on Pulsed Electric Fields Applications in Biology and Medicine (LEA EBAM) and co-organized by COST TD1104 Action (http://www.electroporation.net/).
Finally, we acknowledge the indispensable support of the agencies, societies, and companies who sponsor the school, in particular, the Slovenian Research Agency, the Centre National de la Recherche Scientifique (CNRS), and the Bioelectrochemical Society, which have sponsored the school from its very beginning, and also IGEA (Italy), Mediline (Slovenia), Iskra Medical (Slovenia), BIA Separations (Slovenia), LTFE (Slovenia), and Betatech (France), who made it possible to increase student participation through reduced fees and lodging expenses.
This text is published as Introduction to Fifth Special Issue on Electroporation-Based Technologies and Treatments by Damijan Miklavcic, Lluis M. Mir and P. Thomas Vernier. J Membrane Biol (2015) 248:825–826
DOI 10.1007/s00232-015-9836-3
In this issue (11 articles):
- Editorial
Introduction to Fifth Special Issue on Electroporation-Based Technologies and Treatments
Damijan Miklavčič, Lluis M. Mir, P. Thomas Vernier
Pages 825-826
Download PDF (358KB) View Article - Original Paper
Optimization of the Electroformation of Giant Unilamellar Vesicles (GUVs) with Unsaturated Phospholipids
Marie Breton, Mooud Amirkavei, Lluis M. Mir
Pages 827-835
Download PDF (899KB) View Article - Original Paper
Picosecond and Terahertz Perturbation of Interfacial Water and Electropermeabilization of Biological Membranes
P. Thomas Vernier, Zachary A. Levine, Ming-Chak Ho…
Pages 837-847
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Content Delivery of Lipidic Nanovesicles in Electropermeabilized Cells
P. Henri, R. Ospital, Justin Teissié
Pages 849-855
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Different Cell Viability Assays Reveal Inconsistent Results After Bleomycin Electrotransfer In Vitro
Baltramiejus Jakštys, Paulius Ruzgys…
Pages 857-863
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Mathematical Models Describing Chinese Hamster Ovary Cell Death Due to Electroporation In Vitro
Janja Dermol, Damijan Miklavčič
Pages 865-881
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A Comparative Study on the Effects of Millisecond- and Microsecond-Pulsed Electric Field Treatments on the Permeabilization and Extraction of Pigments from Chlorella vulgaris
Elisa Luengo, Juan Manuel Martínez, Mathilde Coustets…
Pages 883-891
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Protein Extraction by Means of Electroporation from E. coli with Preserved Viability
Sasa Haberl Meglic, Tilen Marolt, Damijan Miklavcic
Pages 893-901
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Efficient In Vitro Electropermeabilization of Reconstructed Human Dermal Tissue
Moinecha Madi, Marie-Pierre Rols, Laure Gibot
Pages 903-908
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Gene Electrotransfer of Canine Interleukin 12 into Canine Melanoma Cell Lines
Ursa Lampreht, Urska Kamensek, Monika Stimac…
Pages 909-917
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Improved Specificity of Gene Electrotransfer to Skin Using pDNA Under the Control of Collagen Tissue-Specific Promoter
Spela Kos, Natasa Tesic, Urska Kamensek, Tanja Blagus…
Pages 919-928
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